Preclinical Antimicrobial Efficacy Studies on IBAL Formulations

Ion Gel ZCM-25 In-vitro Antimicrobial Efficacy Study

  • Completed in 2019 by FDA Certified ARJ Labs, Mexico
  • Ion Gel ZCM-25 had a 100% kill rate in 30 seconds on these human pathogens:
    • Aerobic Mesophilic
    • Fungi and Yeasts
    • Staphylococcus Aureus
    • Pseudomonas Aeruginosa

ION-ZCM1 Redox Potential Assay

  • Completed in 2018 by Exova, USA
  • Oxidation-Reduction Potential was measured at 453.2 mV using method SM2580B

ION-ZC1 Redox Potential Assay

  • Completed in 2018 by Exova, USA
  • Oxidation-Reduction Potential was measured at 532.1 mV using method SM2580B

Ion-ZCM1 Oxygen Radical Absorbance Capacity ORAC Activity Assay

  • Completed in 2018 by the University of Debrecen, Hungary
  • ION-ZCM1 has a TOTAL ORAC = H-ORAC value of 1138 μM TE /100 g
  • Total ORAC value for ION-ZCM1 is 1025 μM TE/ml when measured at a dilution of 12.5%
  • Total ORAC value for ION-ZCM1 is 740 μM TE/ml when measured at a dilution of 6.25%
  • Total ORAC value for Vitamin E is 580 to 585 μmol TE/g at 1:1
  • Total ORAC value for Vitamin C is 128 to 133 μmol TE/g at 1:1
  • Total ORAC value for Pepper Leaf Extract is 64.47 μmol TE/g at 1:50 or approx a 2% solution

ION-ZC1 In Vitro Antimicrobial Study On 12 Hospital Acquired Pathogens Gram-Negative And Gram-Positive Bacterias And Fungus

  • Completed in 2016 by the University of Debrecen, Hungary
  • A non-biofilm antimicrobial assays using broth microdilution-based sensitivity method.
  • All fungal and bacterial strains tested with ION-ZC1 concentrations below 2.125 % v/v observed a 50% turbidity within the 24-hour testing period.

ION-ZC1 In Vitro Antimicrobial Study On MRSA

  • Completed in 2017 by the University of Debrecen, Hungary
  • A non-biofilm antimicrobial assays using broth microdilution-based sensitivity method.
  • The results showed that MIC values for ION-ZC1 against the 10-methicillin-resistant Staphylococcus aureus isolate tested were in the range of 0.212 – 0.85% (v/v).
  • The Control for Vancomycin, MIC values were in the range of 4-16 mg/L.

ION-ZC1 In Vitro Antimicrobial Study On Biofilm

  • Completed in 2018 by the University of Debrecen, Hungary
  • An antimicrobial assays using a microtiter dish sensitivity method.
  • Minimum inhibitory concentrations (MICs) were determined after 24 hours at which a prominent decrease of 50% (average) of biofilm mass was observed over 36 isolates.
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